Figure 3

tap is required for retroelement repression and likely functions with tej . (A) Quantitative RT-PCR (qRT-PCR) showing fold increase in expression of few representative retroelements in tej^48-5, tap¹²⁵ and tej^48-5-tap¹²⁵ mutant ovaries compared to respective heterozygous controls. The tap¹²⁵-mutant displays milder upregulation of TART, I-element, roo and Het-A, whereas the tej^48-5-tap¹²⁵ double mutant exhibits much more severe upregulation of these retroelements compared with both tej^48-5and tap¹²⁵ single mutants. Error bars represent standard deviations. (B) tap-, tej- and the double-mutant testes stained for Ste (green) and DAPI (blue). Top and bottom panels show the apex and posterior regions, respectively. Asterisks mark the hub of the testis. Square brackets denote the distance from the hub to the point where Ste crystals start to appear. Scale bar = 20 μm. (C) qRT-PCR showing increase in ste transcript levels in the tap¹²⁵-mutant testes compared to their respective heterozygote controls, and confirms more severe Ste crystal formation in tej^48-5-tap¹²⁵ double mutants. The error bars represent the standard deviation.