Figure 4

Piwi fails to localize to the nucleus in germline cells of the tej-tap double mutant. (A) Ovaries stained for Piwi (green) and DAPI (blue). Piwi localized to the nucleus in the somatic cells but failed to localize to the nucleus of germline cells of the tej^48-5-tap¹²⁵ double mutants. (B) Western blotting with anti-Piwi antibody showing no significant difference in Piwi expression levels in tej^48-5-, tap¹²⁵- and tej^48-5-tap¹²⁵-mutant ovaries in comparison to their respective heterozygous controls. The same blot was re-probed with anti-α-Tubulin to examine loading. (C) Expression of Myc-Piwi in tej^48-5-tap¹²⁵ ovaries does not rescue nuclear localization of Piwi in germline cells. Immunostaining shows cytoplasmic Myc-Piwi in germline cells of tej^48-5-tap¹²⁵ double-mutant ovaries. Scale bar = 5 μm.