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Figure 5 | BMC Biology

Figure 5

From: The Tudor domain protein Tapas, a homolog of the vertebrate Tdrd7, functions in the piRNA pathway to regulate retrotransposons in germline of Drosophila melanogaster

Figure 5

Tap physically interacts with other components of the ping-pong amplification pathway. (A) Immunoprecipitation with anti-Tap antibody using ovarian lysates followed by western blotting. Vas, Aub, Spn-E, Tej and a trace amount of Ago3 (arrowhead) were pulled down along with Tap. (B-E) S2 cells were co-transfected with FLAG an octa-peptide (DYKDDDDK)-Tap along with V5-Vas, Myc-Spn-E, Myc-Aub, Myc-Ago3, FLAG-Tej and Myc-Tap, and the cell lysates were subjected to immunoprecipitation with anti-FLAG antibodies. IgG was used as control in all IP experiments. (B,C) V5-Vas and Myc-Spn-E were pulled down with FLAG-Tap but not with IgG. (D) Both Aub and Ago3 were pulled down with FLAG-Tap but not with the control IgG. (E) FLAG-Tap was pulled down and blotted with FLAG or Myc antibody. A small amount of Tap precipitated with Tej as well as with itself. Similarly, Myc-Tej was also pulled down with FLAG-Tej. IgG: immunoglobulin G, IP: immunoprecipitation, WB: western blotting.

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