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Figure 6 | BMC Biology

Figure 6

From: The Tudor domain protein Tapas, a homolog of the vertebrate Tdrd7, functions in the piRNA pathway to regulate retrotransposons in germline of Drosophila melanogaster

Figure 6

The tap gene is required for robust piRNA production against germline transposable elements, while tap along with tej is required to maintain piRNA population in germline. (A) Abundance of genome-matching unique reads, 21 to 29 nucleotides in length, in tap125-, tej48-5- and tej48-5-tap125-mutant ovaries and their respective heterozygous controls. The tap mutants showed reduced numbers of 23- to 29-nucloetide small RNAs, but the tej48-5-tap125 double mutants showed the most severe reduction in comparison to both single mutants. (B) Mapping of the 23- to 29-nucloetide sequences to bidirectional piRNA clusters at 20B3 and 42AB, and a single-strand piRNA cluster, flamenco. A feeble reduction in bidirectional cluster mapping piRNAs in tap125 mutants was discernible compared to tej48-5 mutants, while tej48-5-tap125 double mutants had a greater reduction than both single mutants.

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