Nuclear localization of HD-Zip transcription factor GL2. Confocal laser scanning images show Arabidopsis ovules expressing (A) wild-type EYFP:GL2 and (B) EYFP:gl2ΔSTART proteins, indicating nuclear localization in immature mucilage secretory cells. (C) Nuclear stain DAPI (blue), EYFP (yellow) and merge (green) display nuclear localization of EYFP:gl2ΔSTART from (B). (A, B, C) Scale bar: 20 μm. Live imaging of leaf trichomes indicates nuclear localization of (D) EYFP:GL2 and (E) EYFP:gl2ΔSTART, as well as EYFP:GL2-START domain swap proteins containing the (F) mouse StAR (pink) or (G) ATML1 START domains. Domain swaps with (H) EDR2 or (I) REV START domains result in diffuse expression in trichomes. Light (left; green, chlorophyll) and matching fluorescence (right; red, chlorophyll) images are shown for each construct. Scale bar: 100 μm. ATML1, Arabidopsis thaliana Meristem Layer 1; DAPI, 4′,6-diamidino-2-phenylindole; EDR2, Enhanced Disease Resistance 2; EYFP, enhanced yellow fluorescent protein; GL2, Glabra2; REV, Revoluta; StAR, steroidogenic acute regulatory protein; START, StAR-related lipid transfer; YFP, yellow fluorescent protein.