Loss of Jhd2 enhances rDNA silencing at NTS1, but not NTS2, in a Sir2-independent manner. (A) Schematic of an rDNA unit with the position of the mURA3 reporters inserted into NTS1 or NTS2 (see Figure 2A for features). (B, C) URA3-based rDNA silencing assays at either NTS1 or NTS2. WT and the indicated deletion strains in the DMY2798 (leu2::mURA3), DMY2804 (RDN1-NTS1::mURA3) or DMY2800 (RDN1-NTS2::mURA3) background were used. Chr, chromosome; WT, wild type.