Loss of Jhd2 increases association of condensin subunits, Tof2 and Csm1/Lrs4 with the RFB. (A-F) ChIP analyses were performed using WT, jhd2Δ or gis1Δ strains carrying the indicated TAP-tagged proteins as shown in Figure 2B. Quantitation of each ChIP result is shown at the right. Error bars represent the SD of three PCRs performed using two independent chromatin preparations, and asterisks indicate statistically significant differences compared with WT (*, P <0.05; **, P <0.01). IP, immunoprecipitation; WT, wild type.