Processing of tRFs is distinct from miRNAs and tRF-3 and tRF-1 are mostly cytoplasmic. (A) tRF read counts in wild type, dicer1 -/-, and dgcr8-/-mouse ES cells. (B) Same data sets as A, but read counts of various miRNAs are shown. (C) tRF read counts in Drosophila S2 cells either mock, dicer-1 dsRNA, or dicer-2 dsRNA treated. (D) Same data sets as C, but read counts of two miRNAs are shown. (E) tRF read counts from fly heads of either wild type, dicer-2 mutant, or r2d2 mutant flies. (F) tRF reads in wild type or dcr1 delta S. pombe. (G) tRF read counts in HeLa cell nuclear fractionation or whole cell.