Examples of tRF-3-mRNA CLASH chimeras. The most abundant read of 10 of the most prevalent tRF-3-mRNA interactions found in our analysis were analyzed with mfold's RNA Folding Form using default settings. The output of mfold is represented with the tRF depicted 3' to 5' and the mRNA sequence 5' to 3'. For each interaction the number of reads supporting the interaction is shown to the left, and the delta G from mfold is shown to the right. CLASH, cross-linking, ligation, and sequencing of hybrids.