Deletion of MRC1 sharpens the replication profile. A). Correlation matrix of the replication profiles: shown are the Pearson's correlation coefficients measuring the similarity between the replication profiles of wildtype cells (seven biological repeats), mrc1 deleted cells (three repeats) and mutants previously associated with the scaling phenotype (clb5Δ, dpb3Δ, dpb4Δ, dia2Δ, met7Δ, and sic1Δ). Note the low correlation of the mrc1 profile with the profiles of the scaling mutants. B). Replication profiles at chromosome XI. DNA was extracted from FACS-sorted asynchronous S phase population and quantified using high-throughput illumina sequencing. The average DNA content measured at each genomic position is plotted as a function of the chromosomal coordinates. Active origins appear as local maxima. Plotted are replication profiles of mrc1Δ (red) and clb5Δ (green), with wild-type in black. Gray vertical lines represent location of confirmed origins, as defined in OriDB. C). Gene deletion alters the relative origin activation time: the relative activation time of each OriDB-defined origin (f
, peak height of the respective replication profile) was measured from the respective profile and is shown as a function of the relative activation time of the respective origin at wild type, f
(left subplot, red denotes mrc1Δ, green denotes clb5Δ). Left subplot shows the same for local minima. D). Profile sharpness defined by height of local maxima and local minima: Shown are the histograms of the function (fstrain (xi) -fwt (xi)) displayed for each strain (red = mrc1Δ; green = clb5Δ) for local maxima (left) and local minima (right). The respective Boxplots are shown in E) and represent the percentiles 75%, 50% and 25%. E). Boxplot of the data in C). F). Profile sharpness defined by decay of autocorrelation: Plot of the autocorrelation of wildtype, mrc1Δ, clb5Δ, dpb3Δ and sic1Δ in blue, red green, green and green, respectively. FACS, fluorescence-activated cell sorting.