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Figure 3 | BMC Biology

Figure 3

From: Global identification of Smad2 and Eomesodermin targets in zebrafish identifies a conserved transcriptional network in mesendoderm and a novel role for Eomesodermin in repression of ectodermal gene expression

Figure 3

Smad2 and Eomesa bind common regulatory elements proximal to ndr1 -responsive genes and regulate the developmental functions of Nodal signalling. (A) De novo motif analysis identifies the known Smad binding element (SBE) and consensus T-box binding site within Smad2 ChIP-seq peaks and Eomesa ChIP-seq peaks. (B) Venn diagram indicating the overlap between Smad2 and Eomesa ChIP-seq peaks. (C) Venn diagram of the overlap between genes with proximal Smad2 and Eomesa binding (within ±10 kb of their TSS). (D) Examples of genes with common proximal peaks of Smad2 and Eomesa binding; scale in reads per million reads. (E-G) Functional and anatomical analysis of genes showing Smad2 and/or Eomesa binding. Colour coded as in C. (H) Comparison of ndr1-responsive genes with subsets of genes with proximal Smad2 and/or Eomesa binding as defined in C. ** P P ≤2 × 10-3; **** P ≤1 × 10-105. ChIP-seq, chromatin immunoprecipitation sequencing; TSS, transcription start site.

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BMC Biology

ISSN: 1741-7007

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