Features of the RMP pir multigene family A) Phylogenetic tree of RMP pir s , showing the different clades (S1 to 8, L1 to 4) and separation of the `long' (L) and `short' form (S) pir s. B) Features of the different RMP pir clades. For each clade we show the total number of RMP pirs followed by their distribution (pie charts) in the three species and the distribution of gene lengths (box plots). In addition, pie charts show the relative abundance of clades in the different isolates/lines of P. chabaudi and P. yoelii. The expression bar plots (red bars) visualise the expression of the pirs of different clades in the different life cycle stages (except for the ookinete stage since expression/FPKM values are below the cut of level of 21). A pir is assigned to a life cycle stage based on the highest FPKM value. The height of the expression bar represents the percentage of all pirs in that clade. FKPM, fragments per kilo base of exon per million fragments mapped; RMP, rodent malaria parasites.