RT-PCR confirmation of extended 3'-UTR at PCL5 . A) IGV profiles for the PCL5 locus, with red arrow denoting the 3'-UTR extension. B) Schematic of locus as in Figure 3, showing the downstream tDNA, with RT-PCR primers designed to detect the 3'-extension. Results again confirm extended transcripts in tfc6 and B-box mutants. Wild type and tfc6 strains are the same as in Figures 2 and 3, and the B-box mutants are DDY5124 and DDY5126. IGV, integrative genomics viewer.