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Figure 4 | BMC Biology

Figure 4

From: Airway branching has conserved needs for local parasympathetic innervation but not neurotransmission

Figure 4

Laser ablation denervates the lung, resulting in fewer endothelial cells and reduced proliferation. Sections from control ablated lung (A) and nerve ablated lung (B) were stained for phosphohistone H3 (pink), neural β3-tubulin (cyan), and VEGFR2 (green). Neural ablation reduces neural cells to 6% (C) and cell proliferation to about 30% (D) of control ablated samples but with no difference in apoptosis (E) measured by cleaved caspase 3 staining (mean, SEM). Compared to control ablations, neural ablation reduces endothelial cell numbers by two-thirds (F) and reduces their cluster size (G). Scale bars = 50 μm. Error bars represent S.E.M. For all plots, n = 3 samples, with 10 or more sections analysed per treatment. SEM, standard error of the mean; VEGFR2, vascular epithelial growth factor receptor 2.

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