Independent variable

d.f.

SS

MS

Fvalue

Pvalue


Homozygosity

1

0.069

0.069

0.569

0.456

Reproduction system

2

2.349

1.175

9.643

0.001

Error

33

4.019

0.122





Linkage disequilibrium

1

0.034

0.034

0.209

0.650

Reproduction system

2

1.391

0.0696

4.309

0.022

Error

31

5.005

0.161





 Table shows the two analyses of covariance done to test if the fitness responses at generation 35 (Δw_{
t
}) are explained by SNP homozygosity (1Ho) or pairwise SNP linkage disequilibrium (r^{2}) at generation 22 (see also Figure 5 and Methods for details on statistical models and calculations of genetic diversity). In each model, reproduction system (Trioecy, Monoecy, Androdioecy) is treated as the fixed independent variable. SS and MS stand for SumofSquares and MeanofSquares, respectively. Error degrees of freedom (d.f.) for the model testing the effects of linkage disequilibrium is 31 because two monoecious populations from the sudden regime had such low SNP diversity that r^{2} could not be calculated (see Additional file 1: Figure S4).