HDACi do not alter the properties of generated NPCs. (A) Neurospheres were digested and plated on dishes. NPCs were then visualized using immunostaining with anti-NESTIN (green) and anti-SOX2 (red) antibodies. (B) The percentages of NPCs were calculated, the error bars indicate SEM; n = 4. (C, D) NPCs were differentiated into neurons, which was detected by immunostaining with anti-MAP2 (green) and anti-NeuN (red) antibodies after 50 days of differentiation. The efficiency of neuronal differentiation was compared among control cells and HDACi treated cells. Scale bars, 50 μm. The error bars indicate SEM; n>/=3. HDACi, HDAC inhibitors; NPCs, neural progenitor cells; SEM, standard error of the mean.