Figure 1

Analyses of fetal, placental and liver weights during mid- to late-gestation. A) At E14.5 Grb10 ^m/+ and Grb10 ^m/+/Dlk1 ^+/p fetuses and placentae were significantly overgrown when compared to wild type and Dlk1 ^+/p fetuses. B) Placental efficiency, calculated as a ratio of fetal/placental mass, was significantly increased at E17.5, but not E12.5 or E14.5, for Grb10 ^m/+ and Grb10 ^m/+/Dlk1 ^+/p conceptuses when compared to both wild type and Dlk1 ^+/p. C) At E17.5 Grb10 ^m/+ and Grb10 ^m/+/Dlk1 ^+/p fetal livers were significantly heavier than both wild type and Dlk1 ^+/p livers. When expressed as a proportion of body weight (relative weights) Grb10 ^m/+/Dlk1 ^+/p fetal livers were significantly enlarged compared to wild type and Dlk1 ^+/p livers. D) Table summarising results of statistical analyses in A-C. All values represent means ± SEM, one way ANOVA with Tukey’s post-hoc analysis. For E12.5 WT n = 23, Dlk1 ^+/p n = 13, Grb10 ^m/+ n = 13, Grb10 ^m/+/Dlk1 ^+/p n = 16 ; for E14.5 WT n = 19, Dlk1 ^+/p n = 22, Grb10 ^m/+ n = 22, Grb10 ^m/+/Dlk1 ^+/p n = 25; for E17.5 WT n = 4, Dlk1 ^+/p n = 8, Grb10 ^m/+ n = 6 Grb10 ^m/+/Dlk1 ^+/p n = 8; * P <0.05; ** P <0.01; *** P <0.001. ANOVA, analysis of variance; E, embryonic day; SEM, standard error of the mean; vs, versus; WT, wild type.