The essential Schizosaccharomyces pombe Pfh1 DNA helicase promotes fork movement past G-quadruplex motifs to prevent DNA damage

Table 2 Distribution of G4 motifs relative to functional elements in S. pombe

Genomic feature	Total number of features	G4 overlap	Expected overlap	G4 motif status	FDR adjusted q-value
Promoters	3,237	45	27	Enriched	<0.003
Dubious ORFs	71	13	4	Enriched	<0.003
3′ UTRs	5,144	48	34	Enriched	0.013
5′ UTRs	5,144	47	27	Enriched	0.003
Nucleosome-depleted regions	2,300	54	18	Enriched	<0.003
Meiotic DSB hot spots	288	112	62	Enriched	<0.003
Protein-coding ORFs	5,144	303	382	Depleted	<0.003

G4 motifs are enriched in promoters of Pol II transcribed ORFs, NDRs, dubious ORFs, UTR and meiotic DSB hot spots. G4 motifs are depleted from ORFs overall, as well as essential and highly transcribed genes. G4 motifs are also enriched in Cdc20 occupied sites in Pfh1-depleted cells (Figure 2), γH2A sites (Figure 3), telomeric repeats and rDNA repeats (Figure 1B).

ISSN: 1741-7007