Skip to main content

Table 2 Distribution of G4 motifs relative to functional elements in S. pombe

From: The essential Schizosaccharomyces pombe Pfh1 DNA helicase promotes fork movement past G-quadruplex motifs to prevent DNA damage

Genomic feature Total number of features G4 overlap Expected overlap G4 motif status FDR adjusted q-value
Promoters 3,237 45 27 Enriched <0.003
Dubious ORFs 71 13 4 Enriched <0.003
3′ UTRs 5,144 48 34 Enriched 0.013
5′ UTRs 5,144 47 27 Enriched 0.003
Nucleosome-depleted regions 2,300 54 18 Enriched <0.003
Meiotic DSB hot spots 288 112 62 Enriched <0.003
Protein-coding ORFs 5,144 303 382 Depleted <0.003
  1. G4 motifs are enriched in promoters of Pol II transcribed ORFs, NDRs, dubious ORFs, UTR and meiotic DSB hot spots. G4 motifs are depleted from ORFs overall, as well as essential and highly transcribed genes. G4 motifs are also enriched in Cdc20 occupied sites in Pfh1-depleted cells (Figure 2), γH2A sites (Figure 3), telomeric repeats and rDNA repeats (Figure 1B).