The essential Schizosaccharomyces pombe Pfh1 DNA helicase promotes fork movement past G-quadruplex motifs to prevent DNA damage

Table 3 G4 motif association with high Pfh1 and Cdc20 occupancy

G4 Status	Pfh1 Status	Number of motifs	Number with Cdc20	% with Cdc20	P -value
G4 with Pfh1	Expressed	90	36	40%	1.5E-17
G4 w/o Pfh1	Expressed	356	14	4%
G4 with Pfh1	Depleted	90	39	43%	1.9E-11
G4 w/o Pfh1	Depleted	356	38	11%

G4 motifs associated with high Pfh1 occupancy are significantly more likely to be associated with replication fork pausing as indicated by high Cdc20 occupancy in both Pfh1-expressing (P = 1.5E-17) (Pfh1 status expressed) and Pfh1-depleted (Pfh1 status depleted) cells (P = 1.9E-11). G4, G-quadruplex.

ISSN: 1741-7007