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Figure 2 | BMC Biology

Figure 2

From: Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii, and its use for gene discovery

Figure 2

PCR analysis of transposon insertions. Linear fragments containing transposon TrpA-cat-Mu and flanking H. volcanii DNA were released from plasmid vectors by restriction digestion and transformed into H295 cells. Four clones from three transformations were isolated, their DNA was extracted, and PCR was used to detect the presence of the inserted transposon. (A) H295/iSKT11 clones were analyzed with primers HSP746 and HSP747. (B) H295/iSKT12 clones were analyzed with primers HSP744 and HSP745. (C) H295/iSKT10 clones were analyzed with primers HSP742 and HSP743 as well as (D) with primers HSP742 and HSP750. White rectangles indicate the 2.2 kb transposon. Flanking DNA from the integrated fragments is shown with purple. The primer recognition sites are shown with arrows. H295 indicates control PCR with DNA from the parental strain. Crtl indicates template-free control reactions. A 1 kb DNA ladder (Invitrogen) was used as a size standard.

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