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Figure 3 | BMC Biology

Figure 3

From: Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii, and its use for gene discovery

Figure 3

Southern analysis of transposon insertion mutants. Genomic DNA (2.5 μg) was digested with EcoRI + KpnI and probed with a transposon-specific probe. Purified TrpA-cat-Mu transposon (15.5 ng) and parental H295 DNA were used as positive and negative controls, respectively. Lanes 1 and 2 represent pigmentation mutants, lanes 3 and 4 auxotrophic mutants, and lanes 5 to 12 randomly selected uncharacterized mutants. Faint bands in lanes 11 and 12 are due to a smaller amount of DNA in these two samples.

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BMC Biology

ISSN: 1741-7007

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