Table 1 Plasmids used in the work

pBlueScript SK+

E. coli cloning vector; Amp^r

Stratagene

pUC19

E. coli cloning vector; Amp^r

[34]

pTA231

E. coli – H. volcanii shuttle vector; Amp^r, Trp⁺

[35]

pTA351

E. coli – H. volcanii shuttle vector; Amp^r, Trp⁺

[36]

pMPH20

Carrier plasmid of transposon TrpA-cat-Mu; transposon released by BglII digestion. Amp^r, Cam^r, Trp⁺

This work

pSKT10

H295 pHV3 bases 228, 564 – 230, 899^a cloned in pBlueScript SK+. TrpA-cat-Mu inserted between HVO_B0199 and HVO_B0200. Amp^r,Cam^r, Trp⁺

This work

pSKT11

H295 chromosomal DNA bases 385, 714 – 390, 107^a cloned in pBlueScript SK+. TrpA-cat-Mu inserted in HVO_0434. Amp^r, Cam^r, Trp⁺

This work

pSKT12

H295 chromosomal DNA bases 1, 530, 448 – 1, 534, 708^a cloned in pBlueScript SK+. TrpA-cat-Mu inserted in HVO_1650. Amp^r,Cam^r, Trp⁺

This work