Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii, and its use for gene discovery

Table 6 Oligonucleotides used in the work

Oligonucleotide	Sequence (5′ to 3′)	Comment
HSP675	GCGCGCGGATCCAGTTATGTGCGTTCCGGATG	Cloning of Pfdx + trpA
HSP683	GCGCGCGGATCCCGTGGATAAAACCCCTCGTT	Cloning of Pfdx + trpA
HSP734	CGTTGTAAAACGACGGCCAGTG	Sequencing from pBlueScript SK+, forward
HSP735	GGGAACAAAAGCTGGAGCTCC	Sequencing from pBlueScript SK+, reverse
HSP742	AACGCCTCGTAGAGCGTGTA	PCR spanning the insertion site of iSKT10. Forward, 5′ at 228,414 of pHV3^a
HSP743	CGTCGTCGAACGTACCTCAT	PCR spanning the insertion site of iSKT10. Reverse, 5′ at 230,985 of pHV3^a
HSP746	TGAGTCGAGACGGAGCGAGA	PCR spanning the insertion site of iSKT11. Forward, 5′ at 1,530,341 of H. volcanii chromosome^a
HSP747	CCCACGAGAAAGGCGAGAAC	PCR spanning the insertion site of iSKT11. Reverse, 5′ at 1,534,771 of H. volcanii chromosome^a
HSP744	GTTTCTTGGCGAGGGGTTC	PCR spanning the insertion site of iSKT12. Forward, 5′ at 385,560 of H. volcanii chromosome^a
HSP745	CGAGACCTTCTCCAGCTCGT	PCR spanning the insertion site of iSKT12. Reverse, 5′ at 390,228 of H. volcanii chromosome^a
HSP750	CCCCGTGGAGGTAATAATTGACG	Sequencing of TrpA-cat-Mu insertion site, PCR of iSKT10 insertion site
HSP751	CGTCGCAACGCCCACCGC	Sequencing of TrpA-cat-Mu insertion site

^aNumbering according to H. volcanii D2 pHV3 (NC_013964.1) and chromosome (NC_013967.1), respectively.

ISSN: 1741-7007