Figure 3

Hypo-responsiveness associated with NCD4lo cells develops exclusively in periphery and is also seen in monoclonal T cells. A. Ex vivo analysis of stained thymic cells to show single positive mature CD4lo and CD4hi cells (log and linear plots), and corresponding forward scatter (right) (one of three experiments). B. Levels of CD5 in log (left) and linear (right) scale from the same experiment as in A. C. 3H-Thymidine incorporation assay to measure proliferation of sorted thymic single positive mature CD4lo and CD4hi cells 60 hours post-activation with anti-CD3 (1 μg/ml) and anti-CD28 (3 μg/ml) (Mean ± SE of triplicate cultures, one of three experiments). D. 3H-Thymidine incorporation at 60 hours of sorted NCD4lo and NCD4hi cells from TCR transgenic OT-II mice to titrating concentrations of OVA-II peptide with irradiated B6 DCs as APCs (mean ± SE of triplicate cultures, one of four experiments). E. Amount of IL-2 estimated from supernatants 48 hours post stimulation. Data from one of four experiments. APCs, antigen-presenting cells; DCs, dendritic cells; NCD4, naïve CD4 T cells; SE, standard error; TCR, T cell receptor.