Figure 5

Consequences of peripheral residence in NCD4 T cells. A. CD4 levels on sorted, and differentially labeled NCD4lo (left and right) and NCD4hi (middle and right) cells prior to (day 0) and four days after transfer. Left and middle panel – log plots, right panel – an overlay in linear scale (one of three experiments). B. CD4 MFI values for NCD4lo and NCD4hi cells seven days post-transfer. Each circle represents an individual recipient mouse and the horizontal line indicates mean ± SE. C. Number of transferred NCD4hi and NCD4lo cells recovered from individual recipient mouse spleens seven days post-transfer shown as mean ± SE (one of two experiments). D. 3H-Thymidine incorporation in OT-II cells parked in WT mice for one or five weeks in response to titrating concentrations of OVA-II peptide. Data normalized for 200 OT-II cells (Mean ± SE of triplicate cultures; 1 of >7 experiments). Horizontal line indicates background cpm. E. Levels of IL-2 in culture supernatants from a representative experiment as in panel D. Horizontal line indicates background IL-2 levels (one of five experiments). F. Histogram overlays showing CD4 levels as log and linear plots of one-week-parked or eight-week-parked OT-II cells. G. CD4 MFI levels from long-parked (>4 week) and short-parked (<2 week) OT-II cells (Mean ± SE, one of six experiments). MFI, mean fluorescence intensity; NCD4, naïve CD4 T cells; SE, standard error; WT, wild type.