Figure 7

Prolonged residence in vivo leads to loss of functionality and accumulation of ROS in NCD4 cells. A. Histogram overlays (plotted on log on left and linear scale on right) for DCFDA from OT-II cells parked for one or eight weeks in WT mice. B. DCFDA MFI values from long-parked (>4 weeks) and short-parked (<2 weeks) OT-II cells (mean ± SE, one of three experiments). C. 3H-Thymidine incorporation in response to titrating concentrations of OVA-II peptide in OT-II cells parked for one or six weeks in WT mice (with or without Euk-134 treatment). Data normalized to 200 OT-II cells (Mean ± SE of triplicate cultures; one of three experiments). Horizontal line indicates background cpm. DCFDA, 2-7-dichlorofluorescin diacetate; MFI, mean fluorescence intensity; NCD4, naïve CD4 T cells; ROS, reactive oxygen species; SE, standard error; WT, wild type.