Figure 9

NCD4lo cells resemble naïve CD4 T cells from aged mice in their phenotypic and functional attributes. A. CD4 levels on ANCD4 and YNCD4 cells (plotted on log on left and linear scale on right) stained as a mixture in a single well. B. Representative forward scatter histograms for ANCD4 and YNCD4 cells. C. Comparison of CD4 MFI values of ANCD4 and YNCD4 cells (Mean ± SE; data representative of three experiments). D. Data for forward scatter from individual mice (Mean ± SE; data representative of three experiments). E. Representative data showing levels of cytokines in supernatants of in vitro primed and recalled YNCD4 and ANCD4 cells. F. IFNγ/IL-13 ratios and mean ± SE calculated based on absorbance values. G. Histogram overlays of baseline ex vivo p-Erk (top) and total Erk (bottom) in ANCD4 and YNCD4 cells (plotted on log on left and linear scale on right). H. Pooled data, of paired samples showing baseline p-Erk MFI values (Mean ± SE) in ANCD4 and YNCD4 cells. I. ΔΔCt values of Real time RT-PCR for miR-181a normalized to miR-142 on ANCD4 and YNCD4 cells (Mean ± SE from five sets of independently sorted cells). J. 3H-Thymidine incorporation of ANCD4 and YNCD4 cells treated or not with BCI prior to activation (Mean ± SE of triplicate cultures). Data representative of six mice, one of three experiments. K. IFNγ/IL-13 and IFNγ/IL-4 ratio for YNCD4, BCI treated ANCD4 or BCI untreated ANCD4 cells during recall response. Data representing one of four independent experiments. ANCD4, naïve CD4 T cells from aged mice; BCI, (E)-2-benzylidene-3-(cyclohexylamino)-2,3-dihydro-1H-inden-1-one; MFI, mean fluorescence intensity; SE, standard error; YNCD4, naïve CD4 T cells from young mice.