Figure 2

Lingulodinium does not have rhythm transcripts over a 24-hour cycle. (A) DESeq significant differences for all pairs of samples taken LD 2, 6, 14 and 18 under a light dark cycle were combined to a single list of 131 contigs. (B) Contigs with more than five-fold maximum difference are typically those with lower mean read counts. (C) A heat map of all the significant differences detected shows a preponderance of contigs whose abundance appears greatest at ZT 14. Hierarchical clustering was performed only with rows to preserve the order of the time points. (D) Northern blots using probes prepared from a random selection of contigs indicate the RNA-Seq predicted differences are false positives. (E) Densitometric scans of Northerns relative to actin as a loading control (red) compared to normalized RNA-Seq data (blue). LD, light/dark; ZT, Zeitgeber time.