Entry failure by a toxofilin +tachyzoite. Time lapse of a toxofilin+ RON2mC parasite releasing RON2 to assemble a junction (pink arrowhead) into an HFF cell PM but then encountering a cell swelling area and failing to progress. A RON2 ring marked by a green arrow is concomitantly capped backwards, accumulates at the posterior and is subsequently released into the medium. The two top panels show the DIC and RON2mC (green) fluorescent merged frames while the two bottom panels show the parasite RON2mC signal; all scale bars: 5 μm. DIC, HFF, human foreskin fibroblast; PM, plasma membrane; RON2, RhOptry Neck.