Figure 4

Accurate nucleosome remodeling during nuclear reprogramming. A) qPCR results of the germ layer specific genes showed that their expression is specific in the tissue of origin before reprogramming (top panel), but their expression in the pluripotent cells fails to reflect the tissue of origin of iPSCs (bottom panel). B) Nucleosome occupancy in the four representative genes in A is similar between the ESC R1 and iPSC cell lines. Transcripts from RefSeq are shown under the tracks. More loci are given in Additional file 6: Figure S3. C) A schematic diagram demonstrates the generation of the mouse secondary iPSCs and their nearly indistinguishable nucleosome organizations, irrespective of their tissue of origin. The transcription factor binding sites (short colored lines) were distributed across the genome in a manner consistent with the characteristic topological relationships with nucleosomes, and this distribution was similar between the iPSC cell lines. ESC, embryonic stem cell; iPSCs, induced pluripotent stem cells.