Figure 2
From: A substrate ambiguous enzyme facilitates genome reduction in an intracellular symbiont
Quantitative expression analyses of Buchnera and A. pisum pantothenoate biosynthesis genes. Expression profiles for (A) Buchnera pantothenate biosynthesis genes and (B) complementary A. pisum β-alanine biosynthesis genes were generated for three independent A. pisum lineages (5A, 9-2-1 and LSR1). Expression of Buchnera genes in bacteriocytes (Ba) was normalized to atpE (BU003) while expression of A. pisum genes in whole insects (WI), bacteriocyte (Ba) and gut (G) tissues was normalized to GAPDH (ACYPI009769). Relative abundance of each gene was compiled into a heat map showing high expression (yellow) to low expression (blue). The box shows gene expression fold-change in A. pisum LSR1 bacteriocyte (Ba) tissues relative to whole insect (WI) expression. Fold-change was calculated according to [36]; for each aphid lineage n = 3.