ADAR protein level is sensitive to temperature. (A) Western blot analysis of the HA-tagged dADAR protein, from the loxP control allele as well as the dadar hypomorphic allele, both generated through homologous recombination [32,33]. β-actin is presented as a loading control. Wild type dADAR, which lacks the HA tag is presented as a negative control (−HA). (B) Quantification of western blot analysis. dADAR-HA signal is normalized to β-actin signal from each lane. Bars represent standard deviation. (C) Relative editing at sites that respond to dADAR dosage and/or to temperature. Editing at 20°C (black) is aligned along the black line. Green sites are unresponsive to temperature, but sensitive in the hypomorph (gray), suggesting editing is more dependent on RNA structure than on dADAR level. Purple sites are sensitive to both temperature and dADAR levels, while orange sites are more responsive to temperature than to dADAR level, suggesting at these sites that an additional factor, such as RNA structure, is partially responsible for the response of editing to temperature.