LET-60 Ras in IL1, OLQ and RMD neurons controls the direction of locomotion. (A) The proportion of animals exhibiting CL in wild type, let-60(n2021lf) mutants and transgenic animals in which IL1, OLQ or RMD neurons were inactivated by UNC-103(gf) (n ≥3 assays). (B) Rescue experiments in which the wild-type let-60 gene was expressed in each or all the neurons regulating foraging in let-60(lf) mutants (n ≥3 assays). (C) IL1-, OLQ- or RMD-specific RNAi of let-60 causes frequent CL (n ≥3 assays). (D) Definition of ‘head angle’ as the angle between two lines where one passes the worm centroid and the terminal bulb of pharynx and the other the terminal bulb of pharynx and nose tip. The ventral or the dorsal bends of the head are defined as positive or negative values, respectively. (E) Head angles of ventral and dorsal bends in WT and RMD::let-60RNAi animals. n ≥69 bends. Error bars represent SEM. Asterisks indicate significant differences compared with wild-type animals (A, C, and E) or let-60(lf) mutants (B) (*P <0.05, **P <0.01, ***P <0.001, Dunnett’s test or Student’s t-test). N.S., not significant. CL, circular locomotion; SEM, standard error of the mean; WT, wild type.