LET-60 Ras mediates the localization of GLR-1 glutamate receptors in RMD neurons. (A) The proportion of wild type, glr-1(ky176) mutants and glr-1 mutants with expression of glr-1 gene by its own promoter exhibiting CL (n ≥5 assays). (B) The proportion of wild type, glr-1(ky176) mutants and glr-1 mutants with expression of GLR-1::GFP by rig-5a promoter exhibiting CL (n ≥4 assays). Error bars represent SEM. Asterisks indicate significant differences (*P <0.05, **P <0.01, Student’s t-test). (C)
mgl-1 promoter drives expression in NSM, RMDV, RMDD and AIA neurons. Neurites of RMDD neurons (enclosed by a red rectangle) were investigated by analyzing the clusters of GLR-1::GFP in RMD neurons specifically. (D) GLR-1::GFP clusters expressed by the mgl-1 promoter in wild type (left panel) and wild type with RMD-specific RNAi of let-60 (right panel). Enlarged images show neurites of RMDD neurons (yellow arrowheads = GLR-1::GFP clusters). (E) Average intensity of GLR-1::GFP clusters in neurites of RMDD neurons is significantly reduced by RMD-specific knockdown of let-60 (n ≥10 animals). Error bars represent SEM. An asterisk indicates significant differences (Student’s t-test). (F) Representative images of mgl-1p::GFP in wild type (left panel) and wild type with RMD-specific RNAi of let-60 (right panel). Scale bars = 10 μm. CL, circular locomotion; SEM, standard error of the mean.