A site mapping in tissue datasets. We used the raw sequencing reads to map high-quality polyA sites onto the WS190 worm annotation and compared our results with two published C. elegans 3’UTRome datasets. (A) The number of polyA clusters mapped from polyA-containing sequencing reads (total), the portion of those that mapped to the WS190 worm genome annotation (mapped), the number of genes with polyA sites mapped (closest gene to the polyA cluster) and the number of isoforms resulting from distinct mapping of polyA clusters (isoforms). (B) The majority of mapped 3’UTR isoforms are supported by two published 3’UTRomes and almost 90% of them are supported by at least one dataset. Left panel: the percentage of isoforms mapped to either of two published 3’UTRomes (green and red), to both (blue), and those not present in either 3’UTRome dataset (purple). Right panel: the distribution of 3’UTR length for all 3’UTR isoforms found in each tissue dataset, along with the median (vertical dashed red line) and the average length.