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Figure 7 | BMC Biology

Figure 7

From: The development and maintenance of the mononuclear phagocyte system of the chick is controlled by signals from the macrophage colony-stimulating factor receptor

Figure 7

The effect of chicken CSF1-Fc on blood cell profiles in newly hatched birds. MacRed (CSF1R-mApple) birds were dosed subcutaneously with 50 μg chicken CSF1-Fc or PBS on hatch day (day 0), day 1, day 2 and day 3, then sacrificed 24 hours after the final injection. Blood was collected into heparinized tubes, and separated by density gradient centrifugation as described in Methods. The cells were stained with anti-CD45, anti-CD3 or anti-BU-1, and analyzed by flow cytometry. (A) Representative FACS profiles of marker expression used to calculate the values in (B). (B) Mean monocyte number (transgene-expressing cells) compared to mean thrombocyte number (CD45lo), mean leukocyte number (CD45hi), mean thrombocyte + leukocyte number (CD45+), mean B cell number (BU-1+) or mean T cell number (CD3+) for each treatment group. (C) Panels show representative FACS plots for the peripheral leukocyte populations from non-transgenic wild-type birds, or PBS or chicken CSF1-Fc-treated MacRed birds as indicated, stained for CD45 (upper panels), Bu-1 (middle panels) or CD3 (lower panels). The transgene (mApple) is on the y-axis, detecting the transgenic macrophages. PBS, treated with phosphate-buffered saline; WT, wild-type.

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