Figure 5

T3 transgenic tobacco plants showed diverse patterns of flower opening. (a) Compared to WT tobacco flowers, flowers of transgenic tobacco plants had less corolla coloration, less petal elongation at anthesis, showed typical cleistogamy phenotype or had impaired flower buds. Flowers were collected from T3 tobacco plants at the anthesis stage or shortly after anther dehiscence for those flowers that did not open. Flowers from each transgenic group were arranged in three panels representing three groups of transgenic lines (Cl1, Cl2 and Cl3) generated from three independent T0 plants. Inside each group the lines were arranged in the same way present in the X-axis of graph b. (b) The level of PpJAZ1 transgene was quantified in leaves of each transgenic line using the qRT-PCR approach. The gene expression in each line was normalized to that of NtActin and was calculated relative to the gene expression of a reference sample (the one that had the lowest expression). The results represent the mean ± SE of three replicates prepared from different leaves of the same plant. (c) Scatter plot matrix generated based on the data recorded for 25 transgenic and three WT tobacco plants. r is calculated using Pearson correlation coefficient. WT, wild type.