Figure 3

Myc directly binds to and controls Uhg promoters. A) ChIPseq profile for the Uhg1 locus in S2 cells. Labeling conventions are as in Figure 1A. B) ChIP-qPCR of Uhg loci. Chromatin was isolated from naïve S2 cells or from S2 cells depleted of Myc, precipitated with rabbit anti-Myc (Santa Cruz) or control IgG antibodies and assayed by qPCR for enrichment of the indicated loci. Similar results were obtained with mouse anti-Myc antibodies (not shown). C) Activity of CG5033- and Uhg1-luciferase reporters in S2 cells upon Myc-overexpression or Myc-knockdown. As a control for dsRNA against Myc (Myc-kd), dsRNA against GFP was used (ctr-kd); for Myc-overexpression tubulin-GAL4 was co-transfected with UAS-Myc, and control samples were transfected with tubulin-GAL4 and UAS-T (empty vector). Error bars show standard deviations from six transfections. *P <0.01; **P <0.005 (Student’s two-tailed t-test).