Classification of stages and levels of jasmonates in developing stamens of wild type and jai1-1. (a) Inflorescences of wild type and jai1-1 showing the six developmental stages defined for this study. (b) Developmental stages of dissected stamen cones of wild type and jai1-1. (c) Jasmonate levels in developing stamens. Stamens of the respective stages were extracted and contents of OPDA, JA, and JA-Ile were determined. Mean values ± SD are shown. Data of the same developmental stage were compared between wild type and jai1-1 by Student’s t-test (*P ≤0.05, **P ≤0.01, ***P ≤0.001, n ≥3). JA, jasmonic acid; JA-Ile, JA-isoleucine; OPDA, cis-12-oxo-phytodienoic acid; SD, standard deviation.