Figure 2

Carbohydrate binding by CL-K1. A) Glycan-array analysis of CL-K1. Key: open circle, mannose; closed circle, galactose, closed square, N-acetylglucosamine; closed diamond, N-acetylneuraminic acid; open square, N-acetylgalactosamine; open diamond, glucose; and triangle, fucose. High-mannose and blood group/Lewis antigen ligands are in green and blue. B) Inhibition of CL-K1 binding to immobilized yeast invertase (10 μg per well) by high-mannose epitopes (Man2: α1-2 mannobiose; Man3: α1-3, α1-6 mannotriose; Man5: α1-3, α1-3, α1-6 Mannopentaose). Values are the mean ± error from duplicate measurements. C) Schematic representation of the mammalian high-mannose glycan (Man₉GlcNAc₂). Boxes show the two Man₃ core structures that together form the Man₅ core. CL-K1, collectin-K1.