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Figure 7 | BMC Biology

Figure 7

From: Molecular basis of sugar recognition by collectin-K1 and the effects of mutations associated with 3MC syndrome

Figure 7

Destabilisation of variant CL-K1s caused by the failure to bind to Ca2+. A) Fluorescence spectra of variant CL-K1 trimers in the presence (solid line, 2 mM Ca2+) and absence of Ca2+ (dotted line, 2 mM EDTA) and in 6 M urea (dashed line). The λem, max of Ca2+ bound and Ca2+ free forms of the wild-type fragment are indicated by vertical dotted lines. B) Unfolding of the variant CL-K1 (lower) fragments in urea. All experiments were performed in the presence of 2 mM Ca2+ and data were recorded at 345 nM. Values are mean ± error from duplicate experiments. The denaturation concentrations of Ca2+-bound and Ca2+-free forms of the wild-type fragment are indicated by vertical dotted lines. C) SDS-PAGE gels (17.5%) showing proteolysis of variant CL-K1 fragments with two-fold serial dilutions of trypsin (highest concentration is 4% w/w). CL-K1, collectin-K1; EDTA, ethylenediaminetetraacetic acid.

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