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Table 1 Kinetics of binding of CL-K1 to glycoproteins

From: Molecular basis of sugar recognition by collectin-K1 and the effects of mutations associated with 3MC syndrome

Glycoprotein

k on

k off

K D

(M −1 s −1 )

(s −1 )

(nM)

Mannan

1.6 ± 0.3 × 103

<1.0 × 10−6

<0.8

1.0 ± 0.2 × 106

3.0 ± 0.2 × 10−4

0.31 ± 0.9

Invertase

6.8 ± 0.4 × 104

1.2 ± 0.2 × 10−3

18 ± 4

6.7 ± 0.5 × 105

<1.0 × 10−6

<0.002

gp120

1.5 ± 0.3 × 105

1.1 ± 0.2 × 10−3

8 ± 4

>1 × 106

<1.0 × 10−6

<0.001

  1. All data were fitted to a two-complex binding model. Values of k on above 1 × 106 M−1 s−1 and k off below 1.0 × 10−6 s−1 exceed the reliable detection limits of the instrument, so are given as lower and upper limits respectively. Data are mean ± error from two experiments.