Figure 2
H2O2 stabilizes Lif transcripts after TNF induced gene expression in rMC-1 Müller cells. rMC-1 cells were treated with tumor necrosis factor-alpha (TNF, circle) for one hour to induce Lif gene expression. Following TNF treatment cells were either (A) serum deprived (SD, square) or (B) incubated with ActD (square). H2O2 (50 μM) was added either at the one hour (+ H2O2, triangle) or the two hour time-point (+ late H2O2, del). RNA levels of Lif and Ttp were determined by real-time PCR at the indicated time-points and expressed relative to levels at 0 hour. Shown are means ± SEM of N = 4. One-way ANOVA with Sidak’s posttests were used to compare RNA levels against levels after SD or ActD treatments at two hours and three hours. (*) P <0.05, (**) P <0.01, (***) P <0.001. ActD, actinomycin D. ANOVA, analysis of variance; SEM, standard error of the mean.