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Figure 3 | BMC Biology

Figure 3

From: Long-range regulatory interactions at the 4q25 atrial fibrillation risk locus involve PITX2c and ENPEP

Figure 3

Long-range chromatin interactions in the mouse Pitx2 genomic locus. (A) Schematic representation of a 300 kb region of the mouse genome syntenic to human 4q25. The approximate locations of regions orthologous to human AF1-AF7 are indicated by pink boxes (af1 to af7; af3, in red, is orthologous to human AF3, which contains the lead SNP rs2200733). The promoter regions from which anchor primers for 3C were generated are indicated (Pitx2ab, blue; Pitx2c, red; Enpep, green). Genomic HindIII fragments tested for their interaction with anchor promoter primers are represented by black horizontal bars (1 to 10; F and R denote primer design). (B-D) Normalised 3C interactions, expressed as crosslinking frequencies (y-axis), between the test fragments and the promoters of Pitx2ab (B), Pitx2c (C) and Enpep (D) in atria (red) and ventricle (blue). In each graph, the highest crosslinking frequency values were set to 1. Genome coordinates (x-axis) are from the mouse NCBI37/mm9 assembly. Statistical significance was assessed following one-way ANOVA test of Student-Newman-Keuls. ***P <0.001. Error bars represent ± SEM. ANOVA, analysis of variance; SEM, standard error of the mean; 3C, chromosome conformation capture.

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