Autonomous DNA transposon in the amoeba Acanthamoeba castellanii that is closely related to the MITEs in P. salinus.
a Pairwise alignment of the flanking sequences of the DNA transposon insertion and a paralogous empty site elsewhere in the A. castellanii genome. Red bar indicates the transposon insertion site. Bold and underlined letters (TA) indicate TSD. The paralogous empty site is evidence of transposition. b The structure of the autonomous DNA transposon in A. castellanii. Triangles indicate TIRs. Stars indicate stop codons in the putative transposase sequence. c Alignment of the ends of the consensus sequence of the MITEs in P. salinus and the ends of the autonomous DNA transposon sequence in A. castellanii, referred to as Submariner_Ac1. TIRs for each element are boxed. Columns in the alignment are shaded when nucleotides are conserved in at least three sequences. RC stands for reverse-complement. The sequence similarity between the TIRs of the P. salinus MITE and the A. castellanii DNA transposon Submariner_Ac1 indicates that the P. salinus MITE could have been cross-mobilized in the viral genome by the A. castellanii DNA transposon