TY - JOUR AU - Wong, Adison AU - Wang, Huijuan AU - Poh, Chueh Loo AU - Kitney, Richard I. PY - 2015 DA - 2015/06/16 TI - Layering genetic circuits to build a single cell, bacterial half adder JO - BMC Biology SP - 40 VL - 13 IS - 1 AB - Gene regulation in biological systems is impacted by the cellular and genetic context-dependent effects of the biological parts which comprise the circuit. Here, we have sought to elucidate the limitations of engineering biology from an architectural point of view, with the aim of compiling a set of engineering solutions for overcoming failure modes during the development of complex, synthetic genetic circuits. SN - 1741-7007 UR - https://doi.org/10.1186/s12915-015-0146-0 DO - 10.1186/s12915-015-0146-0 ID - Wong2015 ER -