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Fig. 4 | BMC Biology

Fig. 4

From: Telomere protein Rap1 is a charge resistant scaffolding protein in chromosomal bouquet formation

Fig. 4

Negative charge of Rap1 Bqt1/2 binding domain is important for functional telomere bouquet. a Alignment of Rap1 protein sequences from different fission yeast species, highlighting a highly conserved area within Rap1 (performed using Clustal Omega; EMBL-EBI, Cambridge, UK). Sp, Schizosaccharomyces pombe; Scr, S. cryophilus; So, S. octosporus; Sj, S. japonicus. b Morphology of zygotic asci of rap1-DD337AA mutant compared to cells expressing wild-type Rap1. Frequency of normal four-spore asci is shown in Fig. 3b. c Mitotic cells expressing Rap1-YFP or Rap1(DD337AA)-YFP (green in the merged pictures) and Taz1-mCherry (red in the merged pictures). Both wild-type Rap1 and Rap1-DD337AA co-localize with Taz1-mCherry. Scale bar equals 5 μm. d Series of frames from films of meiosis. The SPB, telomeres and chromosomes were observed via endogenously tagged Sid4-mCherry, Rap1-YFP and Hht1-Cerulean, respectively. Time count starts from the beginning of filming. Scale bar equals 2 μm. Top image, wild-type cells; and bottom four images, examples of defective meiosis in rap1-DD337AA. Note that rap1-DD337AA meiosis is reminiscent of rap1∆ meiosis shown in Fig. 3b

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