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Fig. 5 | BMC Biology

Fig. 5

From: Lhx8 regulates primordial follicle activation and postnatal folliculogenesis

Fig. 5

Lin28a deficiency rescues Lhx8 flx/flx Gdf9Cre-induced PFA. ad Representative histology of control (Lhx8 flx/flx), Lhx8 conditional knockout (Lhx8 flx/flx Gdf9Cre), Lin28a conditional knockout (Lin28 flx/flx Gdf9Cre), and double Lhx8/Lin28a conditional knockout (Lhx8 flx/flx Lin28 flx/flx Gdf9Cre) ovaries. Double Lhx8/Lin28a conditional knockouts show a diminished number of activated primordial follicles. Anti-Nobox antibodies were used to label oocyte nuclei (dark brown). PF: primordial follicle; aPF: activated primordial follicle. e The Lin28 flx/flx Gdf9Cre (Lin28G9cKO) mice have a similar phenotype as control (Ctrl) mice and the primordial follicle count is expressed as the percentage of total follicles. f Quantitation of activated primordial follicles in control, Lhx8 conditional, and double Lhx8/Lin28a conditional knockouts. The percentage of primordial follicles (PF) and activated primordial follicles (aPF) is shown. Three pairs of ovaries from Lhx8 conditional knockout and double Lhx8/Lin28a conditional knockouts mice were serially sectioned, and every fifth section was counted. g AKT phosphorylation is diminished in double Lhx8/Lin28a conditional knockouts. We quantitated by fluorescence p-AKT (T308) expression in the primordial and activated primordial follicles of the Lhx8 flx/flx Gdf9Cre (G9cKO) and Lhx8 flx/flx Lin28a flx/flx Gdf9Cre (G9dKO) ovaries at PD7. There was a significant decrease in AKT phosphorylation in Lhx8 flx/flx Lin28a flx/flx Gdf9Cre primordial and activated primordial follicles compared to Lhx8 flx/flx Gdf9Cre. Student’s t-test was used to calculate P values. *P < 0.05, **P < 0.01. Scale bars: 50 μm (A–D)

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