Fig. 6

Cell cycle arrest at G0/G1 phase in E13.5 Apc CKO lung. a Significant reduction of cell division in E13.5 Apc CKO lung, indicated by increased cells that retain EdU DNA labeling at E11.5. Increased nuclear size (insert) was apparent in Apc CKO lung. b Immunostaining for PH3 and acetyl-α-tubulin, markers for metaphase and G0/G1 phase, respectively. c FACS analysis of DNA contents for the cells isolated from E13.5 Apc CKO or WT control lungs. d Expression of key genes involved in regulation of cell cycle and senescence, detected by real-time PCR. *P < 0.05, n = 4. e Protein levels of the related genes were detected by western blot