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Fig. 4 | BMC Biology

Fig. 4

From: Shell neurons of the master circadian clock coordinate the phase of tissue clocks throughout the brain and body

Fig. 4

The phase and amplitude of clock gene rhythms in central tissues is influenced by photoperiod. a Double-plotted rhythms in Per2 mRNA expression were measured with qRT-PCR for the cerebellum (CB), hippocampus (HIP), olfactory bulb (OB), and septum (SEP) under LD12:12 (blue symbols) and LD20:4 (red symbols). White and black bars on the abscissa represent lighting conditions. n = 3/time-point/photoperiod. *LD12:12 versus LD20:4, LS Means Contrasts, P <0.006. Cosinor analyses of Per2 rhythms are shown in Additional file 1: Table S3. b Summary plots of photoperiod-induced changes in the phase of Per2 rhythms in central tissues. Tissues are ordered by the magnitude of the difference in peak time. Dashed vertical lines indicate the magnitude of the shift displayed by the SCN shell and SCN core in vivo, as detected with PER2 immunohistochemistry (c.f., Additional file 1: Table S4). *Significant phase shift different from 0 h, one sample t-test, P <0.05. c Amplitude of core clock gene expression is reduced in all four tissues. *Student’s t-test, P <0.05. Clock gene rhythms for all four tissues are illustrated in Additional file 1: Figure S3

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